Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TIPARP in samples. An antibody specific for TIPARP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIPARP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIPARP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIPARP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TIPARP, encodes a deduced 657-amino acid protein that shares 91.8% and 52.7% sequence identity with mouse and fugu Tiparp proteins, respectively. The proteins in all 3 species contain 3 highly conserved domains: a WWE (trp, trp, glu) domain, a PARP (poly(ADP-ribose) polymerase)-like catalytic domain, and a novel TPH (TIPARP homologous) domain.
The N-terminal part of the TPH domain contains a CCCH-type zinc finger. Katoh and Katoh (2003) noted that the deduced proteins of 2 human genes, which they called FLJ226093 (PARP12) and ZAP (ZC3HAV1), contain the same 3 conserved domains and share 27.5% and 26% overall sequence identity with TIPARP, respectively.
